Varroa jacobsoni mites that differ in their reproductive success on the European honey bee (Apis mellifera) display differential gene expression
Our lab has sequenced and assembled for the first time a de novo transcriptome of the parasitic mite V. jacobsoni using pools of mites from seven different samples. We also performed a preliminary differential gene expression (DE), analysis of the transcriptomes derived from six of the seven biological replicates of V. jacobsoni populations that differ in their reproductive success on A. mellifera.
Using the edgeR package for the DE analysis we found 1013 differentially expressed genes (FDR < 0.05), between the mites reproducing in A. cerana and the mites reproducing in A. mellifera, of which 93% were up regulated in A. mellifera. We searched for orthologous genes in public databases (Blast/NR, GO and UniProt) and we found that only 698 of the 1013 differentially expressed genes were associated with a functional description, such a biological regulation, metabolic process, molecular binding categories and methyltransferase activity.