Progress toward a genomic sequence of the flesh fly, Sarcophaga crassipalpis

The flesh fly, Sarcophaga crassipalpis exhibit the alternative developmental state of diapause that is induced by the simple environmental cue of a circadian-gated long scotophase (> ~12h) during a 4 day developmental period. There has been extensive research detailing physiological, hormonal and some molecular differences between diapause and nondiapausing states. To continue the molecular examination of the changes during diapause requires a basic description of the possible protein coding sequences of this organism. Recent technological advances has made this project possible. Using the 2014 version of Illumina Mi-Seq, we are sequencing the genome of the flesh fly. After the draft assembly, annotation software will be used to examine the genetic sequences for transcripts involved in the developmental state of diapause.