A reduced-representation genomic sampling approach to detect artificial selection in Aedes aegypti L

Laboratory selection can be a powerful tool in understanding how natural selection operates in nature.  Specifically, hypotheses about natural selection can be tested by examining changes in the genome in laboratory populations and compared to wild populations.  To investigate this approach, we sampled the Aedes aegypti genome in four laboratory lines: Key West, FL origin selected and control and Tucson, AZ origin selected and control, using a double-digest restriction enzyme assisted DNA sequencing (ddRADseq) technique.  We examined the populations for differences between origins (Key West vs. Tucson) and selected versus control lines.  These results suggest this technique may be viable for making connections at a genomic level between laboratory selection experiments and natural selection in wild populations.