Lifecycle and CO1 gene analysis of black oak gall wasp (Callirhytis ceropteroides) on Cape Cod and Martha's Vineyard

Black oak, Quercus velutina, is the dominant deciduous tree on Cape Cod and Martha’s Vineyard. In recent years, oaks on the cape and the islands have experienced severe canopy loss due to the infestation of the black oak gall wasp (Callirhytis ceropteroides). Stressed soil conditions and previous winter moth exposure are two factors that increase oak tree (Quercus spp.) vulnerability. Little is known about the lifecycle of C. ceropteroides and the taxonomy of the species is still unclear. Our research aims to investigate the lifecycle of the black oak gall wasp, specifically emergence patterns, generation time, oviposition location, host specificity and survival rate. We will also perform DNA extraction, PCR analysis and sequence the CO1 barcoding gene for this species. Using the CO1 gene, we hope to determine if C. ceropteroides is the same species that caused extreme canopy loss on Long Island in the 1990’s and possibly determine its native origin. Systemic injections are commonly used to manage other gall wasp species, and may mitigate the black oak gall wasp infestation on Cape Cod and Martha’s Vineyard. We will evaluate the efficacy of trunk injections of emamectin benzoate and imidacloprid as control agents of the gall wasp. Our research may lay the foundation for future biological control efforts and will help arborists and landowners make future management decisions.

* Will have results by end of summer