Identification of wild collected mosquito vectors of diseases using gas chromatography-mass spectrometry in Jazan Province, Saudi Arabia

The cuticular hydrocarbon profiles of eight adult mosquito species using gas chromatography-mass spectrometry were analyzed. Wild collected fourth-instar larvae were reared and single, newly emerged, unfed adult females were used for the analysis. Repeated analysis of variance (ANOVA) and Tukey HSD Post Hoc test was used to test for quantitative differences in relative hydrocarbon quantity. In addition, a linear regression model (LM) was applied using Enter method to determine the diagnostic peaks for the 8 mosquito specimens. The ANOVA test indicated that relative peaks were quantitatively significant (P <0.05). Also, seven compounds showed qualitative differences among the five mosquito vectors tested.