ESA Southeastern Branch Meeting Online Program

Molecular determination of parasitoids (Encyrtidae:Hymenoptera) populations of the Harrisia cactus mealybug (HCM), Hypogeococcus pungens (Granara de Willink) (Hemiptera:Pseudococcidae) in the Caribbean

Tuesday, March 5, 2013
Hilton Baton Rouge
Alberto Galindo-Cardona , Department of Crops and AgroEnvironmental Sciences, University of Puerto Rico, San Juan, PR
Jose Carlos V. Rodrigues , University of Puerto Rico, San Juan, PR
Amy L. Roda , Center for Plant Health Science and Technology, USDA-APHIS-PPQ, Miami, FL
Matthew Ciomperlik , Center for Plant Health Science & Technology (CPHST),, USDA, APHIS, Plant Protection & Quarantine,, Edinburg, TX
Parasitoid wasps of the genus Leptomastidea (Hymenoptera: Encyrtidae) have been identified as natural enemies of the Harrisia cactus mealybug (HCM), Hypogeococcus pungens (Granara de Willink) in the Caribbean region.  Three populations of Leptomastidea spp. have been identified attacking HCM in the region, and individuals were collected from Barbados, Puerto Rico and Florida. Morphological examinations have been unable to establish taxonomical differences among individuals from these three populations.  Genomic sequences (COI and ITS) from single individuals from these populations were compared to determine their identity and their degree of genetic difference. Haplotypes of the three populations were different and the population from Puerto Rico showed genetic homogeneity, in contrast with those of Barbados and Florida.