Baseline susceptibility of Brazilian populations of Spodoptera frugiperda and Diatraea saccharalis to Vip3Aa20 insecticidal protein

Wednesday, November 13, 2013
Exhibit Hall 4 (Austin Convention Center)
Oderlei Bernardi , Dept. of Entomology and Acarology, University of Sao Paulo/ESALQ, Piracicaba, Brazil
Douglas Amado , Dept. of Entomology and Acarology, University of Sao Paulo/ESALQ, Piracicaba, Brazil
Renan S. de Sousa , Dept. of Entomology and Acarology, University of Sao Paulo/ESALQ, Piracicaba, Brazil
Fabiana Segatti , Syngenta Proteção de Cultivos Ltda., São Paulo, Brazil
Julio Fatoretto , Syngenta Proteção de Cultivos Ltda., São Paulo, Brazil
Tony Burd , Insect Resistance Management, Syngenta, Greensboro, NC
Celso Omoto , Dept. of Entomology and Acarology, University of Sao Paulo/ESALQ, Piracicaba, Brazil
To subsidize an Insect Resistance Management (IRM) program we characterized the baseline susceptibility to the Vip3Aa20 Insecticidal Protein in Brazilian populations of S. frugiperda and D. saccharalis. The bioassay procedure was diet surface treatment with purified Vip3Aa20 protein. The estimated LC50 ranged from 92.38 to 611.65 ng Vip3Aa20/cm2 to S. frugiperda populations (6.6-fold variation) and from 61.18 to 367.86 ng Vip3Aa20/cm2 to D. saccharalis populations (6.0-fold variation). Diagnostic concentrations of 2,000 and 3,600 ng Vip3Aa20/cm2 were defined for monitoring the susceptibility to both S. frugiperda and D. saccharalis populations.
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