Use of microsatellites to genotype crosses of Spodoptera frugiperda and to distinguish isofamilies

Wednesday, November 13, 2013: 4:18 PM
Meeting Room 5 ABC (Austin Convention Center)
Renee Arias , National Peanut Research Laboratory, USDA-ARS, Dawson, GA
Maribel Portilla , ARS-USDA National Biological Control Laboratory, Stoneville, MS
Carlos A. Blanco , USDA-ARS, Stoneville, MS
The feasibility of genotyping Spodoptera frugiperda (J. E. Smith) crosses between Bt resistant and susceptible populations using SSRs was assessed.  Parents and their corresponding progeny (five resistant, five susceptible phenotype) were genotyped using 192 SSRs on three reciprocate crosses of Bt-susceptible and Bt-resistant populations.  Oviposition, mortality and fecundity were evaluated for five pairs of each of the crosses.  Cluster analysis, heterozygosity and phenotype marker association were analyzed.  Also a multi-allele discrimination of isofamilies by markers associated to insect behavior is reported.