Effect of storage method on manure as a substrate for filth fly development

Numerous studies have   been conducted using manure as a substrate for filth fly development.  In these experiments, the manure is   sometimes frozen for use at a later date.  The purpose of this study was to determine the effects of various   manure storage methods on subsequent house and stable fly development.  Dairy cattle and horse manure were utilized   and the following treatments were applied: 1) freshly collected manure   inoculated with eggs, 2) subsamples of fresh manure placed in vacuumed sealed   bags and 3) subsamples of fresh manure placed in freezer bags. Subsamples of   the manure placed in vacuumed sealed bags and freezer bags were stored in   freezers set at -19°C and -80°C.  The   frozen samples were retrieved, thawed, and inoculated with house fly (Musca domestica) eggs and stable fly (Stomoxys calcitrans) eggs 10 and 30 days following freezing.