Molecular and functional characterization of the first tick CAP2b (periviscerokinin) receptor from Rhipicephalus (Boophilus) microplus

Sunday, November 10, 2013: 2:15 PM
Meeting Room 19 B (Austin Convention Center)
Yunlong Yang , Department of Entomology, Texas A&M University, College Station, TX
Prati Bajracharya , Department of Entomology, Texas A&M University, College Station, TX
Paula Castillo , Department of Entomology, Texas A&M University, College Station, TX
Ronald J. Nachman , Areawide Pest Management Research Unit, Southern Plains Agricultural Research Center, USDA, Agricultural Research Service, College Station, TX
Patricia V. Pietrantonio , Department of Entomology, Texas A&M University, College Station, TX
The cDNA of the receptor for CAP2b/periviscerokinin (PVK) neuropeptides, designated Rhimi-CAP2b-R, was cloned from synganglia of tick Rhipicephalus (Boophilus) microplus. This receptor is the ortholog of the insect CAP2b/PVK receptor, as concluded from analyses of the predicted protein sequence, phylogenetics and functional expression. Expression analyses of synganglion and ovary revealed Rhimi-CAP2b-R transcripts. The expression in mammalian cells of the open reading frame of Rhimi-CAP2b-R cDNA fused with a hemagglutinin tag at the receptor N-terminus was confirmed by immunocytochemistry. In a calcium bioluminescence assay the recombinant receptor was activated by the tick Ixodes scapularis CAP2b/PVK and a PVK analog with EC50s of 64 nM and 249 nM, respectively. Tick pyrokinins were not active. This is the first report on the functional characterization of the CAP2b/PVK receptor from any tick species which will now permit the discovery of the physiological roles of these neuropeptides in ticks, as neurohormones, neuromodulators and/or neurotransmitters.