North Central Branch Annual Meeting Online Program

Simplified methods for RNA interference in western corn rootwom

Monday, June 4, 2012
Regents C (Embassy Suites)
Kathryn M. O'Brien , Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE
Haichuan Wang , Entomology, University of Nebraska-Lincoln, Lincoln, NE
Nicholas J. Miller , Department of Entomology, University of Nebraska - Lincoln, Lincoln, NE
RNA interference (RNAi) is an important tool for the functional analysis of genes.  RNAi is known to work for western corn rootworms when the RNA is delivered by either injection or ingestion.  Injection can be a difficult method to introduce RNA.  Neither technique is convenient for knocking down genes in newly-hatching insects.  We are currently developing simple methods for RNAi that can be applied to eggs and neonates.  We are using the laccase-2 gene as our initial target for RNAi because its suppression results in loss of pigmentation, an easily identified phenotype.
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