ESA Annual Meetings Online Program

The assessment of genetic variability among spined soldier bug (Pentatomidae: Hemiptera) populations

Monday, November 12, 2012
Exhibit Hall A, Floor One (Knoxville Convention Center)
Fatima Mustafa , Entomology, University of Nebraska, Lincoln, NE
Muhammad Irfan Ullah , Entomology, University of Nebraska, Lincoln, NE
Kathleen M. Kneeland , Department of Entomology, University of Nebraska, Lincoln, NE
Thomas A. Courdon , Biological Control of Insect Research Laboratory, USDA, Agricultural Research Service, Columbia, MO
David W. Stanley , Biological Control of Insects Research Laboratory, USDA, Agricultural Research Service, Columbia, MO
W. Wyatt Hoback , Biology, University of Nebraska, Kearney, Kearney, NE
John E. Foster , Department of Entomology, University of Nebraska, Lincoln, NE
Spined soldier bug Podisus maculiventris (Say) is a predatory hemipteran found in North America. It is an important biological control agent for agricultural and forest pests. This predatory species mainly feeds on the eggs and larvae of the lepidopteran and coleopteran species. To assess the genetic variability among the populations from different states, the samples were collected from Michigan, Maryland, Florida, Mississippi, and Missouri . The sample populations were evaluated by the amplified fragment length polymorphism-polymerase chain reaction (AFLP-PCR) technique. Four AFLP primer sets were used to generate molecular markers. The binary data were evaluated using Analysis of Molecular Variance (AMOVA) and Nei’s genetic diversity. The analysis showed that the most of the variations lie within populations. These results suggest a need of special consideration of genetic similarities among different populations for integrated pest management (IPM).
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