Mechanical vector potential and microbiota assemblages of Nylanderia sp. nr. pubens (Hymenoptera: Formicidae)

Since 2002, robust densities of Nylanderia sp. nr. pubens have been found in localized infestations in southeast Texas.  This invasive species is a tremendous nuisance, causing electrical equipment failure, and is ecologically dominant.  As the range of this new invasive pest expands, additional human-valued goods and service industries are threatened.  Hospitals and convalescent homes within the current range of this ant have reported infestations.  This is particularly concerning with regards to the potential for this insect to serve as a vector for pathogenic microorganisms.  In this laboratory experiment, cohorts of N. sp. nr. pubens, were exposed to cultures of Escherichia coli (TOP10 laboratory strain labeled with DsRed-Express protein and an ampicillin resistance gene) and allowed to move across adjoining sterile culture plates (LB ampicillin and methylparabin) through a system of various lengths of Tygon^® tubing, separating their nesting chamber from a food source.  Results showed that N. sp. nr. pubens are capable of mechanically vectoring E. coli.  Pyrosequencing was used to determine the microbiota assemblages associated with N. sp. nr. pubens in hospitals and rural landscapes