ESA Annual Meetings Online Program

D0437 Biosynthesis of defense-priming volatiles from Opuntia associated with cactus boring moth (Lepidoptera: Pyralidae) herbivory

Wednesday, November 16, 2011
Exhibit Hall 3, First Floor (Reno-Sparks Convention Center)
Anastasia M. Woodard , Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR
John Hubstenberger , Department of Agriculture, Arkansas State University, State University, AR
Fabrico Medina-Bolivar , Department of Biological Sciences, Arkansas State University, State University, AR
Greg Phillips , Department of Agriculture, Arkansas State University, State University, AR
Travis D. Marsico , Department of Biological Sciences, Arkansas State University, Jonesboro, AR
To further the investigation of cactus-derived volatile organic compounds as a potential control agent of the invasive cactus borer Cactoblastis cactorum (Berg), sterile root and shoot cultures as well as transformed hairy roots are being generated for the eastern pricklypear Opuntia humifusa (Raf.) Raf. The cultures are being evaluated for their ability to synthesize defense priming volatiles associated with cactus boring moths. Various concentrations of plant hormones (e.g., jasmonic acid, methyl jasmonate, and ethylene) as well as insect materials from the native, co-evolved cactus moth Melitara prodenialis Walker (e.g., labial glands, regurgitant, skin, and gut) are currently being tested as elicitors. Here we report preliminary results from the establishment of O. humifusa cultures and analysis of non-elicited and elicited samples of the tissue and culture media.

doi: 10.1603/ICE.2016.56927

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