ESA Annual Meetings Online Program

D0237 Field longevity of a fluorescent protein marker in an engineered strain of the pink bollworm, Pectinophora gossypiella (Saunders)

Tuesday, November 15, 2011
Exhibit Hall 3, First Floor (Reno-Sparks Convention Center)
Michelle Walters , CPHST Phoenix Lab, USDA - APHIS, Phoenix, AZ
Neil Morrison , Oxitec Ltd, Abingdon, United Kingdom
John Claus , CPHST Phoenix Lab, USDA - APHIS, Phoenix, AZ
Guolei Tang , CPHST Phoenix Lab, USDA - APHIS - PPQ, Phoenix, AZ
Caroline Phillips , Department of Zoology, University of Oxford, Oxitec LTD, Oxford, United Kingdom
Robin Young , Department of Zoology, University of Oxford, Oxitec LTD, Oxford, United Kingdom
Richard Zink , CPHST Fort Collins, USDA, Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Fort Collins, CO
Luke Alphey , Oxford University and Oxitec Ltd, Oxford, United Kingdom
Under extreme conditions, with temperatures regularly exceeding 40°C, DsRed provided a reliable and easily screened marker in pink bollworm (Pectinophora gossypiella (Saunders)) in Delta traps held in a cotton field. The molecular marker was extremely durable and was correct in all analyses. These traits would be a valuable asset for the existing pink bollworm SIT programme, but also demonstrate the potential value of DsRed2 and other fluorescent proteins as markers for SIT in general.

doi: 10.1603/ICE.2016.56040

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