The Diachasmimorpha longicaudata entomopoxvirus (DlEPV) is unique in that it replicates within two different insects, Anastrepha suspensa (Diptera: Tephritidae) (host) and Diachasmimorpha longicaudata (Hymenoptera: Braconidae) (parasitoid). It is believed that the virus is not pathogenic to D. longicaudata, but is immunosuppressive within the host. Several DlEPV genes have been sequenced but have not yet been annotated or compared with homologs in the online databases. Some DlEPV genes are unique, having no homologs in other taxa while others, such as those relating to basic nucleic acid function, have homologs in prokaryotes and eukaryotes. This study analyzes the sequences of three nucleic acid binding proteins and compares them to those of Poxviruses and other organisms. The respective DlEPV amino acid sequences were blasted into several online databases, including NCBI and UniProt. The resulting homologs with the lowest e-values and highest bit scores were then aligned using the ClustalW program. Separate alignments were performed with homologs of the Entomopoxvirinae, Chordopoxvirinae, and other relevant organisms. A phylogenetic tree was constructed using the PAUP program for each of the protein sequences. Two DlEPV sequences were highly homologous with the large and small subunits of several poxvirus early transcription factors. The third sequence had no poxvirus homologs, but was around 60% identical to an early translation initiation factor (eIF-4a) from Drosophila melanogaster, a close relative of A. suspensa. This suggests that DlEPV is unique in that it has probably acquired heterologous genes appropriate to its unusual symbiotic/pathogenic lifestyle.