Monday, December 10, 2007 - 10:17 AM

Isolation and characterization of Manduca sexta sterol carrier protein-x (SCP-x) gene

Min-Sik Kim, and Que Lan, University of Wisconsin - Madison, Entomology, 840 Russell Labs. 1630 Linden Drive, Madison, WI

Cholesterol is very important for cellular membranes and ecdysteroids biosynthesis in insects. The sterol carrier protein SCP-x/SCP-2 has been speculated to have functions in intracellular movement of cholesterol and other lipids species. We have isolated the Manduca sexta sterol carrier protein x (MsSCP-x) cDNA. The MsSCP-x has a 3-ketoacyl CoA thiolase and SCP-2 domains. MsSCPx shares 80%, 79% and 64% similarity to Bombyx mori SCPx, Spodoptera littoralis SCPx and Aedes aegypti SCPx, respectively in the conserved domain.

Species 1: Lepidoptera Sphingidae Manduca sexta (tobacco hornworm)