The changing climate will likely alter the profile of solar radiation reaching the Earth’s surface, primarily in the ultraviolet-B (UVB) band of wavelengths. An understanding of the effects that ultraviolet-B radiation has upon the survivorship and development of Anopheles gambiae larvae could beneficially be incorporated into vector population and disease transmission models, and included into pesticide application decisions. We first established that third instar larvae will almost exclusively restrict cannibalism to moribund or dead larvae, thereby excluding cannibalism as a significant source of mortality. We then exposed same-age, same-size third instar larvae to different doses of ultraviolet-B radiation, isolated by potassium ferrioxalate solution, at an average irradiance of 1.6mW/cm^2. The LD50 exposure time was estimated by Probit analysis at 14.1 minutes. Time to pupation was not delayed significantly in survivors, implying variation in susceptibility in this laboratory population.