Monday, December 10, 2007
D0129

Testing the role of Heliothis virescens alkaline phosphatase in Cry1Ac intoxication

Jonathan D. Willis, jdwillis@utk.edu, University of Tennessee, Entomology and Plant Pathology, 2341 Joe Johnson drive, 205 Ellington Plant Sciences Building, Knoxville, TN, Omaththage P. Perera, operera@ars.usda.gov, USDA-ARS, 141 Experiment Station Road, Stoneville, MS, Michael J. Adang, adang@uga.edu, University of Georgia, Dept of Entomology, 413 Bio Sci Bldg, Cedar St, Athens, GA, and Juan Luis Jurat-Fuentes, jurat@utk.edu, University of Tennessee, Entomology & Plant Pathology, 2431 Joe Johnson Drive, 205 Ellington Plant Sciences Building, Knoxville, TN.

We previously reported on the identification of an alkaline phosphatase from midguts of Heliothis virescens that binds Cry1Ac toxin from Bacillus thuringiensis . This phosphatase was also downregulated in larvae from Cry-resistant strains. In this work we test the role of this phosphatase in Cry1Ac mode of action.


Species 1: Lepidoptera Noctuidae Heliothis virescens (tobacco budworm)

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