Monday, December 10, 2007
D0147

Generation and characterization of Aedes albopictus (Asian tiger mosquito) infected with Wolbachia from Drosophila melanogaster

Eunho Suh, Eunho.Suh@uky.edu, Yuqing Fu, yuqing.fu@uky.edu, David R. Mercer, randy.mercer@gmail.com, and Stephen L. Dobson, sdobson@email.uky.edu. University of Kentucky, Department of Entomology, S225 Agricultural Science Center North, Lexington, KY

Dengue transmission requires an extrinsic incubation period (EIP) within the mosquito vector, typically Aedes aegypti or Aedes albopictus . The natural lifespan of most females in the field is less than the EIP. Therefore, reducing the average lifespan of female vectors is an objective to disrupt disease transmission. A recently discovered strain of Wolbachia (popcorn, wMelPop) that over-replicates in Drosophila melanogaster cells can induce cytoplasmic incompatibility (CI) and shortens the average lifespan of adult flies. We introduced wMelPop into Ae. albopictus embryos via microinjection to modify the age structure of adult populations with the aim of preventing transmission of dengue virus. Here we characterize popcorn infections of Ae. albopictus and describe the potential for control in the field.


Species 1: Rickettsiales Rickettsiaceae Wolbachia pipientis (Wolbachia)
Species 2: Diptera Culicidae Aedes albopictus (Asian tiger mosquito)