Symbiotic microorganisms greatly impact the development and fitness of many insects. Solenopsis invicta Buren, the red imported fire ant (RIFA), is an introduced species that is especially dangerous to native species of ant and other wildlife because they are difficult to control, widely dispersed, extremely aggressive and produce large colonies containing single or multiple queens. Bacterial symbionts of the RIFA, such as the Bacillus sp., Thelohania solenopsae, Vairimorpha invictae, Staphylococcus genus, Lactococcus garvieae, and Pseudomonas genus among others, have been discovered and may play a critical role in the biotic processes of this economically important insect. In this study, we determined that a Bacillus species, which is a Gram positive rod-shaped soil bacterium, routinely occurs in the hemolymph of fourth instar nymphs and queen RIFA in Texas. Through PCR and direct sequencing of the 16S rRNA gene, the presence of Bacillus sp., Pseudomonas, and Staphylococcus were determined. One of the goals of this research is to establish whether Bacillus sp. is a facultative or obligate symbiont of the RIFA and if this bacterium can be used as a biological agent for controlling RIFA populations.