Enhancing soybean germplasm by incorporating resistance to the soybean aphid (Aphis glycines Matsumura) is important to reduce yield loss and insecticide costs in soybean production in the North Central States. Resistance to SBA was screened in four lines developed by the MSU Soybean Breeding Program (E06901, E06902, E06905, E06906) compared to a commercial susceptible variety (Dekalb 27-53). Resistant and commercial lines were planted in replicated plots in East Lansing; MI. Selected rows were kept as yield rows while others were designated for sampling and caging. Plots were sampled weekly for SBA using whole-plant counts. Although SBA populations did not reach the threshold of 250/plant, percent infestations and SBA density was lower in resistant lines compared to the commercial susceptible. Under no-choice conditions using plants infested then caged, resistant lines also had lower SBA density than the susceptible line. Lines were also assessed for Japanese beetle ( Popillia japonica) damage where percent leaflet damage was recorded for all lines. More damage was observed on the resistant lines compared to the susceptible check. In a Petri-dish leaflet assay conducted for 14 days, SBA nymphs confined to resistant leaflets took longer time to reproduce, produced fewer nymphs and died sooner than those on the susceptible control. Michigan line E06901 reported to have the highest level of resistance in the field and in the Petri-dish assay. Results of this study will assist the MSU Soybean Program to exploit lines for advanced breeding and commercial release.