In Missouri, little research regarding crenobiology has been conducted in smaller springs, particularly on the aquatic macroinvertebrate communities that exist in these systems. Because these communities have been poorly studied, aquatic insect, amphipod, and isopod assemblages were examined in eight small to medium discharge springs in Missouri's prairie and Ozark regions. The objective of this study was to determine how communities change longitudinally from the spring source. Quantitative samples were taken at 400 m, 200 m, 100 m, 50 m, 25 m, and 10 m from the spring orifice using a surber sampler and mesohabitats associated with the spring orifice were qualitatively sampled using a D-net. Preliminary results show that the diversity of amphipods and isopods was not high, though they composed the majority of individuals taken at many of the springs. In addition, several blind, subterranean amphipods were collected from spring orifices of a number of sampled springs. Preliminary observations from quantitative sampling suggest that generic richness, excluding Chironomid genera, remains relatively stable along the longitudinal gradient.