Coffee berry borer (CBB) is the Worlds most devastating coffee pest causing an estimated of US$500 million annually in losses and control costs. Beauveria bassiana and Metarhizium anisopliae have been employed to control this pest but their slow kill is an important factor constraining their use. M. anisopliae has been modified to express the scorpion toxin (AaIT) in insect haemolymph and this dramatically increased pathogenicity against Manduca sexta and Aedes aegypti. Here, we demonstrate that the recombinant M. anisopliae strain expressing AaIT (MaPcTox) was also dramatically more virulent to CBB. We evaluated several spore concentrations (1x10¹ through 1x10⁷spores/ml) of both wild type and recombinant strains. Results demonstrated that at concentrations of 1x10² and 1x10³ spores/ml, the recombinant strain significantly increased mortality of CBB by 40% and 55%, respectively. The medial lethal concentration (LC50) was reduced by 18 fold and the medial lethal time (LT50) was reduced by 21.5% at the highest concentration evaluated. The LT50 was reached in 2.85 ± 0.08 days, a significant finding since it is the first occasion that an entomopathogenic fungus killed the CBB in less than 3 days. In future research we will insert the AaIT gene in B. bassiana and compare its efficacy against CBB.