We monitored predators and parasitoids attacking soybean aphid (Aphis glycines Matsumura) in summer (soybean fields) and overwintering (buckthorn bushes) habitats of soybean aphid in 2005 and 2006. Potted soybean plants infested with soybean aphid were used to periodically sample aphid enemies in these two habitats between May and October. Our objective is to assess habitat affiliation and seasonality associated with aphid enemy species occurring in the contrasting soybean aphid habitats. The preliminary assessment of 2005 data indicated that predatory flies (Cecidomyiidae and Syrphidae) generally occurred at similar seasonal abundances between soybean fields and buckthorn bushes, while parasitoids (Aphelinidae and Braconidae) were found mostly in soybean and not buckthorn. Results from 2005 and 2006 will be presented.