Tuesday, December 11, 2007 - 3:59 PM
1060

cDNA sequence, gene organization, and expression of a membrane bound alkaline phosphatase gene from Heliothis virescens.

Omaththage P. Perera, op.perera@ars.usda.gov, USDA-ARS, Southern Insect Management Research Unit, 141 Experiment Station Road, Stoneville, MS, Michael J. Adang, adang@uga.edu, University of Georgia, Dept of Entomology, 413 Bio Sci Bldg, Cedar St, Athens, GA, and Juan Luis Jurat-Fuentes, jurat@utk.edu, University of Tennessee, Entomology & Plant Pathology, 2431 Joe Johnson Drive, 205 Ellington Plant Sciences Building, Knoxville, TN.

Full length cDNA of a membrane bound alkaline phosphatase from tobacco budworm, Heliothis virescens, was cloned and nucleotide sequence was determined. Nucleotide sequence and the organization of the Hv-mALP gene were determined by sequencing direct and inverse PCR products. Quantitative PCR was used to determine the expression levels of Hv-mALP in the foregut, midgut, hindgut, fat body, and muscle tissues.


Species 1: Lepidoptera Noctuidae Heliothis virescens (tobacco budworm)

See more of Ten-Minute Papers, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology
See more of Ten Minute Paper (TMP) Oral

See more of The 2007 ESA Annual Meeting, December 9-12, 2007