Colorado potato beetle (CPB) is a major pest of potatoes in the United States. Although potato has been identified as the preferred host for CPB, other solanaceous crops such as eggplant, tomato and pepper serve as suitable hosts. Geographically separated populations of CPB have shown intraspecific variation to host species. Consequently, field and laboratory choice tests were conducted to better understand host plant choice of CPB in Virginia. In laboratory olfactometer studies, field collected L. decemlineata showed a preference for potato over both eggplant and tomato foliage, and eggplant over tomato foliage. However, field studies using counts of live beetles on untreated paired plants and counts of dead beetles on imidacloprid treated plants found no significant preference for potato over eggplant, but a significant preference for eggplant over both tomato and pepper. To determine if feeding adults affected host plant choice, an imidacloprid treated eggplant or potato plant was paired with an untreated eggplant or potato plant covered in a mesh bag containing two adult male beetles. Significantly more adults were attracted to eggplant with feeding male beetles paired with another eggplant than any other treatment combination. These results indicate that the presence of L. decemlineata on plants may affect host plant choice.