DuPont Rynaxypyr™ (DPX-E2Y45) is a novel anthranilic diamide insecticide that has excellent efficacy on many pests in the order Lepidoptera. Translaminar insecticidal activity was determined for Rynaxypyr™, adjuvants, and other commercial standards using diamondback moth, Plutella xylostella (L.), larvae placed on leaves of cabbage (Brassica oleracea var. capitata L.). Feeding damage ratings were used to determine if mortality was the result of caterpillars coming into contact with treated material or material that had not been treated, with the latter classified as translaminar activity. Analytical methods were used on leaves of several crops to determine the proportion of Rynaxypyr™ on the surface and within the leaf.