DuPont Rynaxypyr™ (DPX-E2Y45) is the first active ingredient from a novel class of insecticides, the anthranilic diamides. Rynaxypyr™ acts selectively as a potent agonist of insect ryanodine receptors and has proven effective against a wide range of lepidopteran pests, as well as additional key pest species within the orders Coleoptera, Hemiptera, Diptera, and Isoptera. In this work, plant uptake and translocation of Rynaxypyr™ from both soil and foliar treatments were evaluated. Compound movement was determined using phosphor imaging with radiolabeled Rynaxypyr™ and quantitative analysis by LC/MS/MS. Corresponding insecticidal activity in distinct plant parts at different time points post-treatment was determined by means of insect feeding bioassays. As predicted by its physico-chemical properties, Rynaxypyr™ was shown to have good xylem mobility, but no measurable phloem movement. In treatments applied to the root zone, root uptake and uniform distribution throughout the plant occurred readily. Results from extensive field research have provided further evidence that Rynaxypyr™ is readily taken up by the roots and translocated to the foliage. This characteristic offers potential for the development of soil application methods to control foliar pests with Rynaxypyr™.