Genetic effects on honey bee division of labor have been identified in different studies. Recently effect of particular genes on honey bee behavior has been elucidated. In this study we focused on one gene, Pgm, important in energy allocation. We compared Pgm genotype frequencies of foragers, soldiers that are of similar age as foragers, and the general colony bees. We found that heterozygote individuals were over-represented in the sample of foragers engaged in energy-demanding flight activity in comparison to other bees. This result was repeated for genetically distinct bees from three different honey bee subspecies found in Turkey.