Since their arrival in 1994, the Africanized bees represent the main local population of honeybees in Puerto Rico. The European bees are brought from the continent, and represent the mainland population. Reproductive isolation exists in Africanized bees? to avoid breeding in the species?. In Apis sp. the males (drones) and the reproductive females (queens) show specific mating flight times within each species during the day. In an island bees can show changes in mating behavior because of the genetic isolation that exists from mainland. Our objectives are to examine the origin and to compare the mating behavior in bees (Africanized vs. European) in Puerto Rico. We predicted that Africanized genes are diluted in the local populations. We compared the flight time of mating of Africanized queens and European drones