Varroa destructor Anderson & Trueman (Acari: Varroidae) is a serious ectoparasitic mite of the honey bee, Apis mellifera L. We explored microwave radiation as a new control method to reduce the chemical resistance and contamination. We used a 2450 MHz commercialized microwave oven to test the sensitivity of adult bees, pupae, and Varroa mites to microwave radiation. Adult bees including foragers and nurse bees have extremely high sensitivity to microwave, over 50% worker bees (n=100) died after 4 s microwave treatment. Pupae had a higher tolerance to microwave radiation but the mortality of pupae was still twice as high as mite mortality. In the reproductive phase mite experiment, we increased the microwave treatment duration to 15 s, both the mortality of mite and pupae were high. The water content of mites (70.1%) is similar to adult workers (69.59% for foragers, 76.54% for nurses) and pupae (77.81%). Therefore water content cannot explain the lower thermal sensitivity of mites. It is possible that the mites are either too small to receive significant microwave radiation, or that their surface to volume ration is much higher for easier heat dissipation.. In conclusion, our results suggested that commercialized microwave radiation is not an appropriate method to control Varroa mite in bee colonies.