Progress in this project includes in vivo production for PoGV, and successful field testing of PoGV and Bt under field and storage conditions. Field studies showed that although PoGV does not immediately kill potato tuber worm (PTM) larvae, it controls future generations by preventing breeding, because infected larvae completing larval development fail to pupate. For example, in 2006 weekly virus treatments in field cages caused a 76.3% reduction in mined leaves, and a 96.3% and 97.4% reduction of PTM larvae recovered from foliage and artificially added tubers, respectively, in the 2nd generation compared with controls. In storage studies, we have tested PoGV and Bt incorporated with various dry carriers (sand, talc, diatomaceous earth and kaolin) as a method to control PTM in stored tubers. Tubers can alternatively be dipped in test suspensions and dried prior to storage. Our data show applications were very effective against neonates at very low rates (e.g. 1 larval equivalent of PoGV can treat over 100 kg tubers), but less effective against larvae already inside tubers, which requires higher rates to kill larvae. In general a successful strategy for these microbials would be to prevent the spread of any suspected infestation in storage. One advantage is that these agents persist for long periods of time under cool and dark conditions of storage. This latter strategy is currently being tested in scaled up studies, including an evaluation of the effect of incubation temperature.