Sunday, December 9, 2007 - 3:47 PM
0247

Comparison of a lipoxygenase knock-out mutants and their near-isogenic wild type corn lines in host preference study of rice weevils

Christian Nansen, cnansen@ag.tamu.edu1, Michael Kolomiets, kolomiets@tamu.edu2, and Xiquan Gao, kolomiets@tamu.edu2. (1) Texas Ag Exp Station, Entomology, 1102 E FM 1294, Lubbock, TX, (2) Texas A&M, Plant Pathology & Microbiology, College Station, Texas 77843, College Station, TX

A series of choice experiments were conducted in which single 9-lipoxygenase gene knock-out mutants and their near-iosgenic wild type corn lines were examined in terms of attractiveness to rice weevils (Sitophilus oryzae), when the insects were offered small samples of kernels in test tubes. While choice experiments involving these mutant and wild type lines have been published elsewhere (i.e. behavioral responses of nematodes to the roots of these lines), this is the first study of how stored grain insects appear to respond to single gene modifications on a kernel level. We show that disruption of a single lipoxygenase gene results in increased attractiveness of rice weevils to the mutant seed. The possible mechanisms of such alteration in weevil's behavior is discussed.


Species 1: Coleoptera Curculionidae Sitophilus oryzae (rice weevil)