In the New World phlebotomine sand flies are represented by 3 genera: Warileyia, Brumptomyia, and Lutzomyia. Until now, most of the taxonomic studies on Lutzomyia have focused on species or groups of species known to transmit pathogens to humans, such as Leishmania or Bartonella. Other taxa of sand flies have mostly been ignored. Two existing classifications propose relationships among the main subgenera and groups of Lutzomyia based on the analysis of morphological characters, one by Young and Duncan (1994) and another by Galati (1998). Fragments of the 18SrDNA, 28SrDNA, and 12SrDNA sequences of Lutzomyia species from 5 countries – Colombia, French Guyana, Peru, Ecuador, and St Kitts (West Indies) ¬– were analyzed phylogenetically. The phylogenetic reconstructions confirm previous observations that Galati's morphologically based cladistic classification of the Luztomayia early diverging lineages best fit the molecular data. They also indicate that an overall revision of the Lutzomyia nomenclature is strongly supported.