Monday, December 10, 2007 - 2:50 PM
0669

Remote sampling and collection of arthropods for low temperature scanning electron microscopy

Ethan Kane, Ethan.C.Kane@aphis.usda.gov, USDA-APHIS-PPQ-PSPI, National Identification Services, 4700 River Road, Unit 52, 4D-04A.3, Riverdale, MD, Eric Erbe, erbee@ba.ars.usda.gov, USDA-ARS, Soybean Genomics and Improvement Lab, Henry A. Wallace, Beltsville Agricultural Research Center-East, Bldg. 465, Entomology Road, Beltsville, MD, and Ronald Ochoa, ron.ochoa@ars.usda.gov, Systematic Entomology Laboratory, USDA-ARS-PSI, PSI, ARS, USDA, 10300 Baltimore Ave. Bldg. 005, Room 137, Beltsville, MD.

Traditional scanning electron microscopy (SEM) has long been useful for imaging morphological features of hard-bodied organisms, however, this technique has not proven practical for many soft-bodied taxa because specimen preparation requires chemical fixation, dehydration, and critical point drying. Recently, low-temperature field emission SEM has been used to obtain high resolution images of a wide range of soft-bodied arthropod taxa, including mites, spiders, collembola, myriapods, as well as a diverse array of both larval and adult insects. Utilizing contact-freeze immobilization techniques, the authors were able to capture and image specimens in near-natural positions, providing insights into both behavior and ecology.