The 2005 ESA Annual Meeting and Exhibition
December 15-18, 2005
Ft. Lauderdale, FL

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Sunday, December 18, 2005
D0535

Detection of genetic variation in populations of Anastrepha ludens (Diptera: Tephritidae) by molecular-based fingerprinting methods

Roxanne E. Garza, roxanne.e.garza@aphis.usda.gov1, Raul A. Ruiz, rur132@psu.edu2, Maria D. Cadena, maria.d.cadena@aphis.usda.gov1, Donald B. Thomas, dthomas@weslaco.ars.usda.gov3, and Don C. Vacek, don.c.vacek@aphis.usda.gov1. (1) USDA-APHIS-PPQ-CPHST, Pest Detection, Diagnostic, and Management Laboratory, Moore Air Base Bldg. 6414, 22675 N. Moorefield Rd, Edinburg, TX, (2) Pennsylvania State University, Entomology, 501 ASI Building, University Park, PA, (3) USDA, ARS, 2413 E. Hwy 83, Weslaco, TX

The detection of DNA polymorphisms in seven populations of Anastrepha ludens (Diptera: Tephritidae) from Mexico and Central America are currently being investigated by polymerase chain reaction (PCR)-based fingerprinting methods. These methods explore variation in microsatellite DNA and include inter-simple sequence repeat-PCR (ISSR-PCR), primer pair ISSR-PCR (pp-ISSR-PCR), and simple sequence repeat-PCR (SSR-PCR). Currently, a small-scale screening is being performed on all ISSR and SSR primers to determine their potential for population variation. Thirty individuals from each of the seven populations will be screened on those primers which show high potential for population discrimination.


Species 1: Diptera Tephritidae Anastrepha ludens (Mexican Fruit Fly)
Keywords: Polymerase chain reaction (PCR), DNA

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COMMENTS AND CORRECTIONS

  • From Silvia Lanzavecchia, Dr, IGEAF INTA Argentina, March 10, 2009
    Dear Roxanne I would like to ask you about the markers you described in the summary and if you have a recent publication on these topics, meaning ISSR and microsatellites in Anastrepha ludens or other related species Sincerely, Silvia