Mosquito populations associated with equine West Nile Virus cases in southeastern Georgia were sampled in 2003 and 2004. Over 10,500 mosquitoes representing over 20 species were collected at 25 sites using CDC light traps and vacuum aspirators. Culex nigripalpus and Cx. erraticus were the most abundant and widespread mosquitoes collected; Cx. quinquefasciatus was rarely collected. More than 8,500 mosquitoes in 411 pools were tested for the presence of West Nile Virus, Eastern Equine Encephalitis Virus, and Saint Louis Encephalitis Virus. No pools were positive for these viruses. Almost 500 blood-fed specimens were collected, and results of blood-meal identifications are pending.