Brood is a positive stimulus for pollen foraging behavior in honey bees. Long-term selection for the amount of stored pollen has resulted in two strains differing in many traits including foraging behavior. High pollen-hoarding strain bees are specialized for collecting pollen and low strain bees are specialized for collecting nectar. We investigated a potential mechanism for these differences, the responsiveness of the high stain bees and low strain bees to brood stimulus. We asked: (1) Do the strains still respond to brood stimuli after more than 20 generations of bidirectional selection? (2) If the strains do respond, do the differences in their responses to brood stimuli explain their foraging behavior differences? (3) Do the strains respond differentially to changes in brood stimuli? Bees from both strains were co-fostered in common “wildtype” colonies with either brood or no brood, the experimental treatments. Pollen and nectar load weights were measured from returning foragers. Bees from the brood treatment collected heavier pollen loads than those from the no brood treatment. High strain bees collected heavier pollen loads and lighter nectar loads than low strain bees in both treatments. Strain differences in the “no brood” treatment demonstrate that traits differing between the strains are not due solely to differential responsiveness to brood stimuli. There was an interaction between genotype and treatment for pollen load weight, indicating that high strain bees responded to the brood stimulus while the low strain bees did not respond.