The striped cucumber beetle, Acalymma vittatum (Fabricius), (Coleoptera: Chrysomelidae) is an important pest of cucurbit crops in Minnesota, USA. However, the overwintering capacity of this pest in temperate regions is poorly understood. In this study, the in-field survivorship of A. vittatum was examined over three consecutive winters. In addition, the supercooling point of A. vittatum was determined as an index of cold hardiness for adults collected from Minnesota, Iowa, and Colorado. At the end of each overwitnering experiment, no survival was observed. The mean supercooling point varied significantly through time in Minnesota and Iowa, with an increasing trend from summer to winter months. Such a trend in supercooling points is generally attributed to freeze-tolerant insects. However, a comparison of the supercooling point to soil temperatures did not allow us to confirm the survival strategy (i.e., freeze tolerance versus freeze intolerance) of this insect during winter conditions. In addition, our result demonstrated that the supercooling point is a poor indicator of cold hardiness for A. vittatum.