Hongmei Li, hongmei_li@entm.purdue.edu1, Regis A. Festucci-Buselli1, Aloisio S. Carvalho-Dias1, Max de Oliveira-Andrade1, Cris Caixeta-Nunes1, Jeff J. Stuart, stuartjj@purdue.edu1, William Muir, bmuir@purdue.edu2, Michael E. Scharf, mescharf@ifas.ufl.edu3, and Barry R. Pittendrigh, pittendr@purdue.edu4. (1) Purdue University, Department of Entomology, 113 WSLR, West Lafayette, IN, (2) Purdue University, Department of Animal Sciences, Lilly Hall, 915 W. State Street, West Lafayette, IN, (3) University of Florida, Entomology & Nematology, bldg 970, Natural Area Drive, Gainesville, FL, (4) Purdue University, Entomology, 901 W. State St, West Lafayette, IN
The over-expression of two P450 genes, Cyp6g1 and Cyp12d1 are associated with the Rst(2)DDT locus (loci) in Drosophila melanogaster. From northern and western blot analysis we observed the expression pattern of these two genes in two DDT susceptible (Canton-S and 91-C) and three DDT resistant strains (Wisconsin, 91-R, and Hikone-R). The CYP6G1 protein was constitutively expressed throughout development in Canton-S and 91-R strains, but was not expressed in third instar larvae of the Wisconsin strain unless the larvae were exposed to DDT. CYP12D1 protein was only expressed in the adult stage. Cyp12d1 mRNA was induced in DDT resistant strains post-exposure to DDT and the expression patterns of Cyp12d1 mRNA varied across DDT resistant strains.
Species 1: Diptera Drosophilidae
Drosophila melanogaster (fruit fly)
Keywords: resistance, insecticide
