Candice A. Stafford, staffc01@ucr.edu and Gregory P. Walker, gregory.walker@ucr.edu. University of California, Entomology, College of Natural & Agricultural Sciences, 4 Temporary Laboratory, Riverside, CA
In the arid and semi-arid regions of the western United States, curly top virus, transmitted by beet leafhopper, causes major losses in several crops including sugar beets, tomatoes, peppers, spinach, and beans. Feeding behavior of beet leafhopper on young sugar beet and tomato plants was studied using a DC electrical penetration graph (EPG). Main feeding behaviors of interest include initiation and termination of probing, xylem and phloem ingestion, and salivation. Correlations between EPG waveforms and specific feeding behavior components were determined using a number of techniques. Simultaneous EPG and high magnification video recordings were used to correlate EPG waveforms with initiation and termination of probing. Honeydew excretion observed in these recordings provided clues regarding ingestion waveforms. To determine if the waveforms were associated with phloem or xylem ingestion, leafhopper stylets were severed by high frequency radio microcautery during the waveform of interest. The portion of plant containing the severed stylets was histologically examination to determine placement of the stylet tips within the plant tissue. The site of ingestion was further determined by the presence of fluorescent markers, transmitted in either the xylem or phloem, in leafhopper guts using an epifluorescent microscope. After completion of these waveform correlations, EPGs can be used to determine when and how beet leafhopper inoculates and acquires curly top virus, and aid in the development of resistant crops and better pesticides that inhibit the inoculation phase of feeding.
Species 1: Hemiptera Cicadellidae
Neoaliturus tenellus (beet leafhopper)
Keywords: Curly top virus
Recorded presentation