Seep fed streams provide a unique habitat for aquatic macroinvertebrates, having a smaller seasonal change in temperature and generally predictable year round flow compared to other first order streams. Our study stream is located within the Grindstone natural area, within the city limits of Columbia Missouri, and like many such habitats is potentially threaten by increased development and recreational use. The goal of this study is to characterize species composition in association with water quality parameters such as nutrients, temperature, pH, and alkalinity. At monthly intervals, starting December 2004, macroinvertebrates were sampled in a standardized manner with a D-frame dip net at 4 stations along the entire reach of the stream. Additionally, water samples were taken from each station for further analysis. Data loggers at each station recorded hourly temperatures. As one might expect, seasonal temperature change was much less marked at the seep than at the month. This stream contains the only known local populations for Cordulegaster obliqua (Say), Amphinemura delosa Harper and Hynes, and Diplectrona modesta Banks. It is hoped that the baseline data presented in this study will provide impetus for further work, for example, how increased development and recreational use effect resident macroinvertebrate populations.