Determining the location of grapevines (Vitis vinifera L.) with Pierce’s disease (PD) within vineyards has been a major question for growers and researchers. Field census has been the only reliable way to identify grapevines infected with PD in the vineyard. Censuses, however, are not cost effective especially when PD incidence is high. In these situations, sampling programs that accounts for the spatial structure and pattern of PD in the vineyard are needed. We characterized the within-vineyard spatial distribution patterns of PD in the lower San Joaquin Valley and the Coachella Valley in California with geostatistics and spatial analysis with distance indices (SADIE). These analyses showed an incident-dependant spatial distribution of PD; no consistent spatial structure when incidence was < 0.1%, spatial trends at low PD incidence (ca. < 0.5%), random patterns at ca. < 2% PD incidence, and spatial aggregation at ca. > 3% PD incidence. When PD was aggregated in vineyards, spatial correlation between two grapevines existed within 26 m. These spatial characteristics of PD distribution are crucial for the development of PD monitoring strategies.