Dolichoderus mariae is a common ant throughout eastern North America. This project examines two aspects of its natural history in north Florida: (1) spatial distribution of nests and colonies and (2) nest architecture. This ant can be found in the upland longleaf pine flatwoods in the Apalachicola National Forest. This habitat is characterized by a sparse overstory of pines and a rich understory of various herbs and forbs. D. mariae is polydomous and makes subterranean nests under wiregrass (Aristida stricta Michx.), which is a common grass and is generally thought to be an indicator of a healthy pine forest. Spatial distribution surveys revealed the nests are distributed in a patchy manner and are highly clumped. The ants excavate soil beneath wiregrass plants to form a roughly conical chamber that leaves the roots of the wiregrass exposed. The exposed roots of the wiregrass provide a scaffold on which the workers and queens arrange themselves and their brood. Workers and queens cluster themselves together and form a dense mat. Each nest contains up to 175 workers per queen. Workers forage primarily from semi-permanent foraging trails. This ant tends a variety of homopterans on surrounding flora that supply them with honeydew.