Locomotor activity patterns of isolated honey bee queens as well as queens accompanied by an entourage of four workers were monitored for several consecutive days within rectangular, clear plexiglas enclosures under controlled laboratory conditions. The enclosures contained bee candy on one extreme end and diluted honey on the other. Activity was measured either in small enclosures with one infrared sensor positioned halfway down its length or in longer enclosures containing a linear array of four sensors. Queens exhibited a wide variety of activity patterns. Most were diurnal, although many showed significant nocturnal components. The typical queen pattern contained several relatively short (20-60 min) bursts of activity separated by longer durations of apparent inactivity. When the queen was attended by an entourage, the resultant activity of the entire group was nearly continuous and showed no evidence of the queen-typical extended periods of inactivity. The queen locomotor patterns will be compared with those of young (circadian arrhythmic) and older (rhythmic) workers.