Eight homologs of vaccinia virus (vv) virion component genes, A10L, A28L, D13K, E10R, I7L, L1R, L4R, and a C-terminal portion of A3L, were identified in a shotgun sequence database of the Diachasmimorpha longicaudata entomopoxvirus (DlEPV) genomic DNA. Phylogenetic analysis and amino acid (aa) pairwise comparison of putative proteins encoded by these genes were performed in relation to homologs in the genus B entomopoxviruses (EPV), Amsacta moorei (Am) EPV, Melanoplus sanguinipes (Ms) EPV, and Heliothis armigera (Ha) EPV, in chordopoxviruses (ChPVs), and in non-pox large eukaryotic DNA viruses (N-PEDVs) of the Asfaviridae, Iridoviridae, Phycodnaviridae and Ascoviridae. Phylogenetic analysis showed that the DlEPV homologs diverged from the ChPVs earlier than those of the Genus B EPVs. Of the eight ORFs analyzed, only vvD13L, vvE10R, and vvL1R occurred in all N-PEDVs. The DlEPV D13L homolog diverged from an EPV ancestor that arose from a node between ChPVs while N-PEDVs and DlEPV L1R arose at a node between N-PEDVs and the poxviruses. Seven (excluding L4R) DlEPV virion component homologs had similar aa identities with EPVs (27.1-56.6%) as they did with ChPVs (27.6-55.5%). Likewise, the same homologs in AmEPV had about the same aa identities with ChPVs (31.9-50.9%) as they did with DlEPV but exhibited much higher percent identities with MsEPV (45.5-72.6%). Conversely, DlEPV had 3.4-38.6 % and AmEPV had 13.3-37.2% aa identities with the three N-PEDV homologs. Taken together, our analyses indicate that DlEPV is more closely related to the poxviruses than to N-PEDVs. This research was supported by NSF grant IBN 9986076 to POL.