Certain endoparasitoid wasps carry polydnaviruses which are characterized by possessing multiple segmented double strand DNAs as genome. They induce physiological and immunological alterations in lepidopteran insects which enable wasp progeny to survive in the hemocoel of lepidopteran insects. To reveal polydnavirus genes causing those alterations, we sequenced 289 kb of the Hyposoter fugitivus ichnovirus (HfIV) genome. The 66 sequenced segments were from 2 to 7 kb in size and encoded 120 potential open reading frames. There are 63 identified genes that appear to be homologs of other, related polydnavirus genes. Six gene families were evident: repeat element protein, cysteine-motif gene, viral innexin, viral ankyrin, NHv1.2 proteins and coiled-coil gene families. We compare the HfIV genome with other sequenced polydnaviruses, such as Campoletis sonorensis ichnovirus (CsIV) and Microplitis demolitor bracovirus (MdBV) and Cotesia congregata bracovirus (CcBV).