The parasitic wasp Diachasmimorpha longicaudata (Dl) (Hymenoptera: Braconidae) introduces a poxvirus into its host Anastrepha suspensa (Diptera: Tephritidae) larvae during oviposition. The Dl entomopoxvirus (DlEPV) subsequently infects the host’s hemocytes and abrogates the larva’s immune system. Thus DlEPV facilitates wasp egg survival and is essential to it. DlEPV virions abound in the poison gland of all female wasps yet it is unknown how wasp larvae acquire the virus. Here we tested the hypothesis that DlEPV DNA is present inside wasp eggs. We determined by SEM that eggs had virions on their chorion but treatment with triton-x-100 (tx) eliminated virions from all eggs checked. Using PCR and specific primers for the DlEPV rifampicin resistance gene we amplified a fragment of expected size from DNA of triton-x treated eggs. A digoxigenin-labeled DlEPV genomic probe hybridized with DNA from tx-treated eggs. These results suggest that DlEPV DNA, likely, occurs within wasp eggs. Additional experiments are underway to confirm these results and determine the relationship (if any), of the viral DNA with that of the wasp. This research was supported by NSF grant IBN9986076 to POL and a Sigma Xi research grant to LFM.