The Asian longhorned beetle (ALB) (Anoplophora glabripennis), introduced to the US from China, is a highly invasive wood-boring species capable of causing billions of dollars in damage. The larvae of the beetle attack a variety of host trees, feeding first in the phloem tissue, and then moving further into the heartwood as the larvae develop. In order to feed on the nutrient poor, inner wood of the host tree, we hypothesized that the larvae have the ability to digest cellulose into glucose, as a source for carbohydrates. Enzymes that digest cellulose in some insect species have been shown to be of microbial origin, but the source of these enzymes in the ALB have not previously been identified. Through aerobic and anaerobic culturing and isolation of the microbiota found in the gut of larval ALB, and comparing enzyme extracts of these microbes to whole gut extracts, the source of specific cellulase enzymes in the ALB was determined. Extracts were subjected to non-denaturing polyacrylamide gel electrophoresis and activity staining with Congo Red was used to determine protein bands active towards carboxymethyl cellulose. These bands were eluted and further purified using SDS-PAGE and molecular weights were estimated against standards. By comparing the active bands obtained from microbial cultures isolated from the gut to whole gut extracts, the source of specific enzymes that allow cellulose digestion were determined.